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UV VIS Spectrophotometer
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UV VIS Spectrophotometer

UV VIS Spectrophotometer

Hardware Specifications
Item
UV-2600
UV-2700
Photometric system
Double-beam optics
Photometric system
Czerny-Turner mounting Single monochromator Lo-Ray-Ligh grade blazed holographic grating
Czerny-Turner mounting Double monochromator Lo-Ray-Ligh grade blazed holographic grating
Detector
R-928  Photomultiplier
Light source
50 W halogen lamp, deuterium lamp, light source auto position adjustment built in
Setting wavelength range
185–1400 nm
Measurement wavelength range
185–900 nm
220 nm to 1400 nm when the ISR-2600Plus Integrating Sphere Attachment is used.
The ISR-2600Plus Integrating Sphere Attachment cannot be used
Wavelength accuracy
± 0.1 nm (656.1 nm D2), ± 0.3 nm (all range)
Wavelength repeatability
± 0.05 nm
Wavelength scanning speed
Wavelength slew rate: about 14000 nm/min Wavelength scan rate: about 4000 to 0.5 nm/min
Wavelength setting
At 1 nm units for scan start and scan end wavelengths, and 0.1 nm units for other wavelengths
Lamp interchange wavelength
Auto switching synchronized with wavelength; switching range selectable between 290 and 370 nm (0.1 nm units)
Spectral bandwidth
0.1/0.2/0.5/1/2/5 nm L2/L5 (low stray-light mode)
Resolution
0.1 nm
Stray light
Max. 0.005 % (220 nm, NaI) Max. 0.005 % (340, 370 nm, NaNO2) Max. 1 % (198 nm, KCl)
Max. 0.00005 % (220 nm, NaI) Max. 0.00002 % (340, 370 nm, NaNO2) Max. 1 % (198 nm, KCl)
Photometric modes
Absorbance (Abs.), transmittance (%), reflectance (%), energy €
Photometric range
Absorbance: −5 to 5 Abs Transmittance, reflectance: 0 to 100000 %
Absorbance: −8.5 to 8.5 Abs Transmittance, reflectance: 0 to 100000 %
Photometric accuracy
± 0.002 Abs (0.5 Abs) ± 0.003 Abs (1 Abs) ± 0.006 Abs (2 Abs) ± 0.3 %T Measured using NIST930D/NIST1930 or equivalent filter
Photometric repeatability
± 0.001 Abs (0.5 Abs) ± 0.001 Abs (1 Abs) ± 0.003 Abs (2 Abs) ± 0.1 %T
Noise level
0.00003 Abs RMS (500 nm)
0.00005 Abs RMS (500 nm)
Baseline flatness
 ± 0.0003 Abs (200–860 nm), 1 hour after light source is turned ON
± 0.0004 Abs (200–860 nm), 1 hour after light source is turned ON
Baseline stability
 Within 0.0002 Abs/h (700 nm), 1 hour after light source is turned ON
Within 0.0003 Abs/h (700 nm), 1 hour after light source is turned ON
Sample compartment
Internal dimensions: W150 × D260 × H140 (mm) Distance between light beams: 100 mm Maximum optical path length of cell: 100 mm
Dimensions
W450 x D600 x H250(mm)
Weight
23 kg
Operating temperature
15°C to 35°C
Operating humidity
30 % to 80 % (no condensation, less than 70 % above 30 °C)
Power requirements
100 to 240 VAC, 50/60 Hz
Power consumption
170 VA
Software Specifications 
UVProbe Software

Operating System
Windows 7 Professional (64/32 bits)
Data Acquisition Modes
Spectrum, Photometric (quantitation), and Kinetics (time course)
General
  • Multitasking (possible to execute data processing while measurement is being performed)
  • Customizable measurement screen layout (wavelengths, data display font and font size, colors, displayed number of digits)
  • GLP/GMP compliant (security, history)
  • Real-time concentration display
Spectrum Mode
  • Comparison/interactive processing of multiple spectra (Note 2)
  • Storage of all processed data with original data set including a history of all manipulations
  • Spectrum enlargement/shrinking and auto scale
  • Automatic interlink with spectral data processing
  • Annotation on spectrum screen
Data Processing in Spectrum Mode
  • Normalization, Point Pick, peak/valley detection, area calculation
  • 1st – 4th derivatives, smoothing, reciprocal, square root, natural log, Abs, %T conversion, exponential conversion, and Kubelka-Munk conversion
  • Ensemble averaging, interpolation, four arithmetic operations (between spectra, between spectra and factors)
Photometric (Quantitation) Mode
  • Single wavelength, multi-wavelength (includes 1, 2, or 3 wavelengths), spectrum quantitation (peak, maximum, minimum, area, etc. for specified wavelength ranges)
  • K-factor, single-point, multi-point calibration curves (1st, 2nd, 3rd order function fitting, pass-through-zero specification)
  • Photometric processing with user-defined functions (+, − , × , ÷ , Log, Exp, etc. functions, including factors)
  • Weight correction, dilution factor correction, and other corrections using factors
  • Averaging of repeat measurement data
  • Simultaneous display of standard sample table, unknown sample table, and calibration curves
  • Display of Pass/Fail indications
Kinetics (Time Course) Mode
  • Comparison/interactive processing of multiple time-course data (Note 2)
  • Single- or double-wavelength measurement (difference or ratio)
  • Simultaneous display of time-course data, enzyme table, and graphs
  • Enzyme kinetics calculation (for single- or multi-cell)
  • Michaelis-Menten calculations and graph creation (Michaelis-Menten, Lineweaver-Burk, Hanes, Woolf, Eadie-Hofstee), Dixon plot, Hill plot
  • Integrated management of sample information including original data, sample weight, and dilution factors, etc.
  • Event recording such as addition of reagents during measurement
  • Time-course spectrum data processing (same as in spectrum data processing)
Report Generator
  • Preview and print functions for customized formats
  • Layout and editing of templates
  • Quick printing using report templates
  • Auto-printing (spectrum mode)
  • Multi-page printout
  • Insertion of date, time, text, and drawing objects including lines, circles and rectangles
  • Insertion of spectral/quantitative data, method, and history
  • Insertion of headers and footers
  • Specification of graph line thickness (for each module), font style, and size

 UV Validation Software

Inspection Items
 Initialization results log • Wavelength accuracy • Wavelength repeatability • Photometric accuracy • Photometric repeatability • Resolution • Stray light • Baseline flatness • Noise level • Drift (baseline stability)
Features
• Select the items to implement during inspection to confirm the approximate time required for full inspection.
• The system is capable of both wavelength repeatability checks and wavelength accuracy checks using a wavelength calibration filter. If the optional mercury lamp unit is used, wavelength accuracy can be inspected using the low-pressure mercury lamp's bright line.
• The software is capable of resolution inspections using methods defined in the EP (European Pharmacopoeia) and USP (United States Pharmacopoeia) as well as the method for checking the bright line spectral bandwidth.
 


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UV VIS Spectrophotometer

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