Human Dipeptidyl Peptidase ELISA Kit
Product Details:
Assaytype             Sandwich
Format                   96T
Assaytime             3.5h
Reactivity               Human
DetectionMethod               Colorimetric
DetectionRange          0.31~20 ng/ml
Sensitivity                   0.19ng/mL
SampleVolume Required Per Well  100ul

Kitcomponents & Storage
Item
Specifications
Micro ELISA Plate(Dismountable)
8 wells X 12 strips
Reference Standard
2 vials
Concentrated Biotinylated Detection Ab (100x)
1 vial, 120 ul
Concentrated HRP Conjugate (100x)
1 vial, 120 ul
Reference Standard & Sample Diluent
1 vial, 20 mL
Biotinylated Detection Ab Diluent
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
Concentrated Wash Buffer (25x)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
Stop Solution
1 vial, 10 mL


TEST PROCEDURE
1.Add 100 L standard or sample to each well. Incubate for 90 min at 37.
 
2. Remove the liquid.
 
3.Add 100 L Biotinylated Detection Ab. Incubate for 1 hour at 37. Aspirate and wash 3 times.
 
4.Add 100 L HRP Conjugate. Incubate for 30 min at 37. Aspirate and wash 5 times.
 
5.Add 90 L of Substrate Reagent. Incubate for 15 min at 37.
 
6.Add 50 L Stop Solution.
           7. Read at 450 nm immediately. Calculation of results