De Novo Transcriptome Sequencing And Analysis
Product Details:
Over the past few years, the next-generation sequencing (NGS) methods have revolutionized functional genomics research. Due to the comparative high cost of sequencing large and complex genomes, RNA sequencing (RNA-Seq) has emerged as a cost-effective means of rapidly acquiring functional sequence information for non-model systems. RNA-Seq is a powerful tool for simultaneous transcriptome characterization and differential gene expression (DGE) analysis in a single cell type, tissue type, or entire organism under defined conditions [1]. In the past few years, RNA-Seq has helped in decoding transcriptomes of various species, including several plants, birds, insects and animals for which no reference genome is available [1-9]. RNA-seq also provides data about alternative splicing, strand-specific expression, expression of unannotated exons (or genes), and identification of fusion genes in cancer. It also provides gene expression data at a greater sensitivity than microarrays.