Berthold Multimode Microplate Reader
Product Details:

Brand	Berthold
Weight	Approx. 20 kg
Excitation Source	Xenon flash lamp: spectral range 200 - 1000 nm
Wavelength Selection	High quality interference filters
Measurement Technologies	Luminescence BRET/BRET2, NanoBRET(TM) Fluorescence (top) Absorbance UV & VIS
Luminescence	<6 amol/well ATP (96 well)
Fluorescence	<7 amol/well FITC (384sv)
Absorbance	Accuracy better 2 %, Precision better 0.6 %
Dynamic Range	6 orders of magnitude (photon counter) 0 - 3.5 OD (photodiode)
Crosstalk	Low crosstalk due to crosstalk reduction design: 10-6 (black plates)
Dimensions (W x D x H)	391 x 470 x 344 mm
Altitude	Max. 2000 m (above sea level)
Humidity	10 - 80 % non-condensing
Temperature Range	Storage: 0 - 40 DegreeC Operation: 15 - 35 DegreeC
Power Consumption	140 VA
Operating Voltage	24 VDC +-5 %
Power Supply	100 - 240 VAC +-10 % 50 / 60 Hz Class I
Regulations	CE, NRTL
PC Operating System	Windows 10 (32/64 bit)
Interface	USB

Highlights
The Tristar 3 Multimode Microplate Reader provides you with application flexibility for today, tomorrow, and beyond to master your changing plate reading applications:
High-sensitivity Luminescence
BRET
Absorbance (UV/VIS)
Fluorescence
FRET
Broad wavelength range selection from UV through the visible rangeFrom UV absorbance for DNA quantification to measurements in the far red or NIR (in selected configurations), the Tristar 3 is ready for any assay and greatly improves the wavelength range of the older TriStar².
BRET/BRET2 and NanoBRET™ upgradable
All Tristar 3 preconfigured models have the features you need to perform BRET assays: best-in-class luminescence sensitivity and a high quality filter system. To start working with BRET, just order the upgrade for your specific assay: BRET1, BRET2, NanoBRET™ or others.
ONE-4-ALL Optics for uncompromised performance
Our patented ONE-4-ALL optics have been optimised to combine the stability and user-friendliness of a multimodal optical system with the sensitivity and versatility of dedicated optical devices.
ONE-4-ALL optics work for both filter- and monochromator-based applications, so there´s never a compromise in performance in any mode.
 
High-performance filter system - when sensitivity counts
The quick-change filter technology of the Tristar series provides you with the flexibility required to meet your application needs: up to 40 different excitation and emission filters can be easily mounted on exchangeable filter slides.
The filters are characterized by high transmissions properties which can be up to 25-fold that of monochromators. Technologies like Time-Resolved Fluorescence (TRF) can be measured more efficiently with filters.
Furthermore, filters with a large bandwidth are available to analyze fluorophores with wide spectra and all luminescence-based assay requiring to use filters, e.g. BRET, BRET2 and NanoBRET™ assays.
 
Ergonomic design to support your work
The ergonomic design of the system provides full front access for all key operations (e.g. plate loading, filter change and connecting reagents). Reagent vials can be stored in an integrated front compartment, providing easy access and visibility. It contains a removable trough that can be filled with water or ice to keep all reagents cooled. Different reagent tube holders are available to ensure secure handling of valuable reagents. The system has a flat surface on its top, providing enough space to put down a laptop.
Powerful crosstalk control – reliable results
Do you often see a few high-intensity samples surrounded by wells of low-intensity when running your samples?  Then you´re potentially affected by a phenomenon called crosstalk, that can produce wrong results and, hence, wrong conclusions. The crosstalk reduction design of the Tristar 3 does almost eliminate this problem: with a crosstalk of 10-6, you never have to worry about the position of your high-intensity samples in your plate.

JET injectors: highest accuracy, speed and cell-friendliness
The Tristar 3 can be equipped with up to 3 JET injectors with variable volumes in pre-measurement or measurement position. The ultra-fast injection speed of JET injectors eliminates any delay between injection and measurement and enables measurement of fast reactions, e.g. flash luminescence.
Berthold Technologies' JET injectors use Teflon bellows for accurate and fast injections and guarantee most efficient mixing as well as extreme longevity.
Highest accuracy and reproducibility - 98 % accuracy over the entire volume range
Low reagent consumption - Low dead volume.