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- We are one of the trusted sources of DNA sequencer, which is a optical bio tech lab instrument.
- Its basic usability is to automate the DNA sequencing process by analyzing the light signals originating from fluorochromes attached to nucleotides.
- The sequencing is initially done only by the size separation and peak reading.
- There after, the actual sequencing reaction(s), cleanup and re-suspension is performed separately in a suitable buffer must.
- The general DNA sequencers can sequence multiple samples in a batch (run) and perform as many as 24 runs a day.
- The process starts by attaching a fluorescent dye to the DNA strand of interest.
- Then, the laser light outfitted in the DNA sequencer emits at a wavelength that is absorbed by the fluorescent dye attached to the sample DNA.
- Afterward, one or more optical detectors are used to detect the wavelength that the dye fluoresces at. The output of the detector gives the reading.
- However, the output of these machines is not perfect as it may contain reading errors and needs to be processed.